Product Name Alanine Aminotransferase (ALT) Assay Kit, 10× Concentrated (Alanine Method)

Key Advantages

• Cost efficiency for high-throughput labs
• Reduced storage and shipping needs
• Equivalent performance to standard ready-to-use kits
• This 10× concentrate is intended for centralized laboratory or reagent preparation use. A validated SOP with acceptance criteria is provided.

Performance and Assay Parameters (After Dilution – Working Reagent)

• Method: Alanine method
• Wavelength: 340 nm
• Reaction type: Kinetic
• Measuring range: 0–1000 U/L (linear r ≥ 0.9900; ≤50 U/L absolute deviation ≤7.5 U/L; 50–1000 U/L relative deviation ≤±15%)
• Reference range: Male ≤37 U/L, female ≤31 U/L (laboratory-specific ranges recommended)
• Precision: Within-run CV ≤5%; within-run range (R) ≤10%
• Accuracy: Relative deviation ≤±10%
• Main interferences: No significant effect from bilirubin ≤100 μmol/L, hemoglobin ≤2.5 g/L, chyle ≤0.15%, ascorbic acid ≤0.5 g/L, heparin sodium ≤50 IU/mL
• Analyzer compatibility: HITACHI 7020, 7060, 7100, 7170, 7180, 7600, 3100, 3500; LABOSPECT 006/008 AS. Validated on listed analyzers; suitable for open-system biochemical analyzers using ALT (Alanine) methodology.
• Calibration type: Multi-point biochemical calibrator

Preparation Overview

• Concentration factor: 10× (R1 only; R2 supplied as dry powder)
• Recommended diluent: Deionized water
• Required simple steps:
  • Dissolve/dilute components in deionized water with stirring
  • Adjust pH to specified target range
  • Bring to final weight/volume with deionized water
  • Filter through 0.22 µm membrane
• Required accessories: 0.22 µm filtration membrane (WX type)

Storage

• Concentrate conditions: R1 concentrate 2–8°C; R2 dry powder room temperature
• Diluted reagent conditions: 2–8°C, stable for 12 months

Detailed Standard Operating Procedure (SOP) for dilution and preparation is provided with each kit.