Ck-NAC 10mlx10

Ck-NAC 10mlx10

Ck-NAC 10mlx10

Model:

 

Technical Specifications

Intended Use
For the quantitative determination of creatine kinase activity in human serum. This test is used as an aid in the diagnosis and management of myocardial infarction and muscular diseases.

Assay Principle
The eastmed CK assay is based on a series of enzymatic reactions:

  1. Creatine Phosphate + ADP → (CK) Creatine + ATP

  2. ATP + Glucose → (HK) Glucose-6-Phosphate + ADP

  3. Glucose-6-Phosphate + NADP⁺ → (G6PDH) 6-Phosphogluconate + NADPH + H⁺

The rate of NADPH formation is measured photometrically by the increase in absorbance at 340 nm, which is directly proportional to the CK activity in the sample.

Kit Components

  • Reagent 1 (R1): Lyophilized powder in 10 mL vials (10 vials per kit). Contains Hexokinase, Glucose-6-phosphate Dehydrogenase, ADP, AMP, NAC, ApA, Creatine Phosphate, and NADP.

  • Reagent 2 (R2): Liquid buffer in a 100 mL bottle (1 bottle per kit). Contains Imidazole Buffer and Glucose.

Storage and Stability

  • Unopened Kit: Store at 2°C - 8°C protected from light. Stable until the expiration date printed on the label.

  • Reconstituted Reagent 1 (Working Solution): Once reconstituted with R2, the working solution is stable for 7 days when stored at 2°C - 8°C protected from light.

Test Parameters

 
 
Parameter Specification
Method IFCC (Creatine Phosphate)
Sample Type Human Serum (avoid hemolyzed or lipemic samples)
Sample Volume 2 µL (Sample to Reagent Ratio 1:50)
Wavelength 340 nm (Primary)
Temperature 37°C
Assay Type Kinetic

Performance Data

 
 
Characteristic Specification
Measuring Range 24 - 1500 U/L
Linearity r ≥ 0.990
Precision (CV) Intra-assay ≤ 5%
Sensitivity ΔA/min = 0.025 - 0.045 for a 300 U/L CK sample
Blank Absorbance A₃₄₀ₙₘ ≤ 0.500
Blank Rate ΔA/min ≤ 0.002

Reference Intervals*

  • Male: 38 - 174 U/L

  • Female: 26 - 140 U/L
    Each laboratory should establish its own reference ranges to reflect its local population.

Compatible Instruments
The eastmed CK Assay is suitable for use on open-channel clinical chemistry analyzers, including:
Mindray BS-200, Sinnowa D-280, Selectra-E, BIO-A25, Sunshine A8, and other semi-automated and automated systems.

Ordering Information

 
 
Product Name Catalog Number Pack Size
Creatine Kinase (CK) Assay EM-CK-0090 100 Tests

For detailed instructions for use, warnings, and limitations, please refer to the complete package insert.

eastmed • Diagnostic Solutions
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