Optimize EM-200Plus Chemistry Analyzer Guide

Step-by-Step Guide to Calculate Reading Points on the EM-200Plus Instrument

$End Point = ⌈ 18 Total Reaction Time (seconds) ⌉$

Example (Total Protein):
- Reaction Time = 10 minutes = 600 seconds.
- End Point = $18 600 \approx 33.33 \to Round up to 34$ .

Purpose: Measure absorbance before and after R2 addition.
- Start Point: 1 point before R2 addition (17) or 1 point after (19), depending on the test.
- End Point:

$End Point = R2 Point + ⌊ 18 Reaction Time After R2 (seconds) ⌋$

Example 1 (Total Bilirubin):
- Start Point = $18 - 1 = 17$ .
- Reaction Time After R2 = 5 minutes = 300 seconds.
- End Point = $18 + ⌊ 18 300 ⌋ = 18 + 16 = 34$ .
Example 2 (HDL-C):
- Start Point = $18 + 1 = 19$ .
- End Point = 34 (same calculation as above).

Purpose: Measure absorbance during stabilized reaction intervals.
- Start Point:

$Start Point = R2 Point + ⌈ 18 Delay Time (seconds) ⌉$

$End Point = Start Point + ⌊ 18 Measurement Time (seconds) ⌋$

Example 1 (ALT - Double Reagent):
- Delay Time = 1 minute = 60 seconds.
  Start Point = $18 + ⌈ 18 60 ⌉ = 18 + 4 = 22$ .
- Measurement Time = 3 minutes = 180 seconds.
  End Point = $22 + ⌊ 18 180 ⌋ = 22 + 10 = 32$ .
Example 2 (AMY - Single Reagent):
- Start Point = $⌈ 18 120 ⌉ = 7$ .
- End Point = $7 + ⌊ 18 180 ⌋ = 7 + 10 = 17$ .

Rounding: Always round up for delay times and down for measurement times to ensure points fit within the 34-point limit.
R2 Handling: For methods without R2 (e.g., AMY), calculate points directly from the start of the reaction.

Method	Start Point Formula	End Point Formula	Example
1 Point End	N/A	$⌈ 18 Time ⌉$	TP: End = 34
2 Point End	R2 ± 1 (17 or 19)	$18 + ⌊ 18 Time ⌋$	TBIL: End = 34
Kinetic	$18 + ⌈ 18 Delay ⌉$	$Start + ⌊ 18 Time ⌋$	ALT: 22 → 32

By following these steps, you can accurately configure reading points for precise and efficient testing on the EM-200Plus.

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