Operating and Maintaining EM3000T Electrolyte Analyzer

Power-On Procedure

1. Reagent Pack Installation:

  • Ensure the reagent pack is correctly installed in the EM3000T.

2. Pump Tube Placement:

  • Confirm the proper placement of all pump tubes.

3. Electrode Installation:

  • Verify the correct installation and connection of all electrodes, ensuring that the bubbles in the electrode filling solution are positioned above the electrodes.

4. Printer Paper:

  • Make sure the printer paper is loaded correctly.

5. Power-On:

  • Turn on the electrolyte analyzer.

6. Self-Check:

  • Verify that the self-check succeeds (indicated by a green light), indicating that the machine is ready for testing.

Sample Preparation

1. Hemolysis Check:

  • Check that samples are free from hemolysis.

2. Sample Integrity:

  • Ensure that samples do not contain substances that might affect test results.

3. Temperature Adjustment:

  • Allow testing samples to reach room temperature before proceeding.

4. Timely Measurement:

  • Following blood sample centrifugation, initiate measurement promptly.

Calibration Check

1. Slope Examination:

  • Examine the slope of the analyzer.

2. Calibration Access:

  • Access the Service menu, then click on Calibration.

3. Electrolyte Calibration:

  • For electrolyte parameters (K/Na/Cl/Ca/PH) calibration, be prepared to repeat the procedure 2-3 times during the initial installation.

4. CO2 Calibration (if applicable):

  • For models with CO2 parameters, select CO2 Cal. and use AB standard solution for calibration.

5. Calibration Confirmation:

  • Reminder: Only commence sample measurement when all slopes are within an acceptable range, and the results are stable.

Measuring Samples

1. Sample Information Entry:

  • Prior to sample measurement, manually input sample numbers or IDs or use an external barcode scanner for automation.

2. Aspiration Process:

  • In the Input menu, select the sample and move it to the probe for aspiration. The machine will signal when to remove the sample.

3. Analyzer Readiness:

  • In Standby mode, click the Wake Up button to enable the analyzer to automatically clean the tubing or perform calibration to ensure it is in good condition for testing.

Calibration and Factor Adjustment

1. Blood Control Calibration:

  • For calibration using blood control, access the Service menu, click on Factor, and enter the password 525. Refer to the user manual for detailed instructions.

Reagent Pack Volume Check and Replacement

1. Volume Check:

  • In the Service menu, navigate to Calibration to view the reagent pack volume on the display.

2. Reagent Pack Replacement:

  • When replacing the reagent pack, choose to either enter the Service menu, select Calibration, and click Regist to register the new reagent pack, or insert the new pack and power on the analyzer for automatic registration.

Daily Maintenance

1. Below 20 Samples:

  • If the number of samples tested per day is below 20, it is recommended to perform a manual STD calibration before leaving the lab.

2. Above 20 Samples:

  • If the number of samples tested per day exceeds 20, it is advisable to wash the tubing with Clean Solution before leaving the lab. Access Service-Maintenance, select Clean Protein, and initiate the cleaning process.

3. Na Slope Below 50:

  • When the Na slope falls below 50, consider performing Na adjustment using Na Conditioning solution. Access the Service menu, click on Maintenance, and select Na adjust, following the on-screen instructions.

Weekly Maintenance

1. Electrode Maintenance:

  • Check the filling solution volume of the electrodes. If it is below 2/3, refill the filling solution.

2. Crystal Buildup:

  • Inspect the electrodes for any crystallizations. Clean and remove them using a wet cotton ball and then dry the electrodes with a dry cotton ball.

Monthly Maintenance

1. Sampling Needle Cleaning:

  • Clean the sampling needle by clearing the inner wall with a stab needle (a standard accessory) and the outer wall with anhydrous alcohol-soaked cotton to remove proteins and blood.

2. Liquid Distribution Valve:

  • Clean the liquid distribution valve by disassembling it and using tools such as a stab needle, ear-sucking ball, and distilled water.

3. Electrode Channels:

  • Clean the liquid passing channels of the electrodes by disassembling them and the positioner and using soft tools such as a cotton thread and ear-sucking ball.
  • Reminder: Do not use the provided stab needle for cleaning the electrode channels.

Tubings Check

1. Tubing Connections:

  • Ensure secure connections of the tubing.

2. Inner Wall Inspection:

  • Examine the inner walls of each tube for the presence of protein or blood, and perform a cleaning procedure or replace tubes as needed.

Elevate Your Lab with the Cutting-Edge Automated Electrolyte Analyzer

Ready to transform your labor